A minimal lentivirus Tat
نویسندگان
چکیده
منابع مشابه
Delineating minimal protein domains and promoter elements for transcriptional activation by lentivirus Tat proteins.
Lentivirus Tat proteins comprise a novel class of RNA-binding transcriptional activators that are essential for viral replication. In this study, we performed a series of protein fusion experiments to delineate the minimal protein domains and promoter elements required for Tat action. We show that a 15-amino-acid region of equine infectious anemia virus (EIAV) Tat protein, when fused to the GAL...
متن کاملEquine infectious anemia virus tat: insights into the structure, function, and evolution of lentivirus trans-activator proteins.
Equine infectious anemia virus (EIAV) contains a tat gene which is closely related to the trans-activator genes of the human and simian immunodeficiency viruses. Nucleotide sequence analysis of EIAV cDNA clones revealed that the tat mRNA is composed of three exons; the first two encode Tat and the third may encode a Rev protein. Interestingly, EIAV Tat translation is initiated at a non-AUG codo...
متن کاملA transitional endogenous lentivirus from the genome of a basal primate and implications for lentivirus evolution.
Lentiviruses chronically infect a broad range of mammalian species and have been transmitted from primates to humans, giving rise to multiple outbreaks of HIV infection over the past century. Although the circumstances surrounding these recent zoonoses are becoming clearer, the nature and timescale of interaction between lentiviruses and primates remains unknown. Here, we report the discovery o...
متن کاملA reversal of Tat
early 15 years of in vitro experiments showed that the Tat transport system, which spans the thylakoid membrane in chloroplasts, sends proteins unidirectionally inwards, from stroma to lumen. On page 281, Di Cola and Robinson report in vivo evidence from protoplasts showing that a large fraction of the proteins that move part way through the channel end up back in the stroma. The Tat system is ...
متن کاملA minimal Tat system from a gram-positive organism: a bifunctional TatA subunit participates in discrete TatAC and TatA complexes.
The Tat system transports folded proteins across bacterial and thylakoid membranes. In Gram-negative organisms, a TatABC substrate-binding complex and separate TatA complex are believed to coalesce to form an active translocon, with all three subunits essential for translocation. Most Gram-positive organisms lack a tatB gene, indicating major differences in organization and possible differences...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1991
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.65.12.7012-7015.1991